John McCain Modern Progressivism Essay - 655 Words

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Slavery in Literature Essay - 1504 Words

Slavery in Literature Frederick Douglass was born into the lifelong, evil, bondage of slavery. His autobiography, Narrative of the Life of Frederick Douglass, An American Slave, Written by Himself, depicts his accomplishments. The narrative, however, is not only the story of his success. It is not simply a tale of his miraculous escape from slavery. Frederick Douglass narrative is, in fact, an account of his tremendous strides through literacy. He exemplifies a literate man who is able to use the psychological tools of thought to escape the intense bonds of slavery. Hard labor, and deprivation of both physical and spiritual necessities, defined slavery in the south. Frederick Douglass struggled throughout his youth to keep†¦show more content†¦He did not allow the slaveholders to capture his opinions and judgement. He did not allow the slaveholders to destroy his dignity. Douglass successfully climbed the ladder from a position of a powerless slave to a strong, devoted, influential, individual. He did so when he began to divert from the path of ignorance to one of education and power. How did Douglass escape the blinding state of ignorance? How did Douglass manage to escape misery and attain happiness? How did Douglass manage the escape of bondage and slavery? This transition involved a series of processed, the first being the destruction of ignorance. In the eyes of the slaveholders, a happy slave was an ignorant one. For this reason, they were denied any form of knowledge. The masters were fearful of discovering that a slave had run away or were plotting an escape. They were afraid of a slave acquiring a vision beyond the unjust conditions they lived by. 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He exposes and reflects on the truth about slavery and racism during the 1870s, proves how Huck s view has been formed society, includes American characteristicsRead MoreSlave Narratives : A Darker Side Of American History900 Words   |  4 PagesAmericans would not be able to understand about the cruelty of slavery and the stories of the great African-Americans who escaped slavery and became key abolitionists and opponents against slavery. Slave narratives carry a huge importance in American history, because without them we would not be able to understand the extent of what slave owners went to with their â€Å"property†. The slave narrative is defined as a piece of literature written by one who has been enslaved during one point in their lifetimeRead MoreRacial Issues Throughout The Literature Of Colonial America960 Words   |  4 Pages Racial issues today are rooted in the literature of colonial America. 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Impact of Change Management on Organizational Structure-Samples

Question: Discuss about the importance of change that is being undertaken by the organizations for their growth and expansion. Answer: Introduction to change The organizations undertake the changes in order to bring in improvements in their structure. It is based on the requirements of the consumers and for undertaking the organizational progress. The increasing amount of demand and the change in the preferences of the consumers have made it mandatory for the organizations to undertake the changes (Langley et al., 2013). The management of the organization helps in undertaking the changes and facilitates the improvements of the organization. Proper management of the organization has helped the companies in bringing about the change in the market structure. It facilitated the evolution of the organizations in the market based on the change of the demand of the consumers (Kerzner, 2013). The market analysis of the organizations helps in the understanding of the changes that it requires to undertake. Therefore, the changes that the organizations undertake help in bringing in improvements in the structure and the statistics of the organization. The main purpose of the analysis is to evaluate the change management and their impact on the organizational structure through the improvements in the market (Hornstein, 2015). Research on the change management based on the model of change The improvements in the organizational structure are being undertaken through a thorough evaluation of the customer needs. Adhering to the needs and requirements of the consumers helps the organization in making its progress in the market. Globalization has enhanced the market availability of the organizations. Sustainability in the market is the major concern of the organizations and thus the companies are required to undertake constant modifications. According to Cameron Green, (2015), the sustainable approach of the organizations force them in undertaking the change in the market structure. Modifications undertaken by the organizations are based on the changing requirements of the consumers. On the other hand, Doppelt (2017) believed that market competition is the major reason as to why the companies are adopting the changes. The sustenance of the brands and the products in the market is the major concern of the organizations. Therefore, the steps taken by the organization to bring the relevant changes in the structure help in serving the objective of the companies. The challenges that are being faced by the organizations in the market due to the activities of the existing competitors and the new entrants make it an important point for the organizations to undertake the change. Laudon Laudon (2016) believed that the legal factors also affects the growth of the organization and thereby bring in the needs of modifying the policies of the organization. It helps in coping up with the change in the business environments. Flexibility in the structure of the organization helps in the undertaking of the international trade by the companies. It helps in the expansion and growth of the company depending on the change. The application of the Lewins model of change helps the organization in determining the changes based on their organizational needs and preferences of the consumers (Refer to Appendice 1). The Unfreeze stage helps in analyzing the changes that the organization is required to undertake based on the preferences of the consumers. The Change ph ase helps in determining the change and implements the changes in the structure of the organization in order to bring in the favorable outcomes on their investments. It helps the organization in making the suitable modifications that are required to be undertaken in order to maintain the situation of the company in the market. The Change phase helps the organization in serving the objective of the organization relating to its growth and expansion. The Freeze stage comes after the change is being undertaken by the organization. It helps in maintaining the records of the change and thereby make the organizational progress in the market (Shirey, 2013). It helps in sticking on to the decision taken by the management relating to the change and thereby makes it the culture of the organization. It helps in maintaining the organizational stability in the market. The flexibility of the structure of the organization helps in bringing in the changes in the organization and thereby modify based on the needs of the consumers. On the other hand, the changes that the companies must undertake helps them in undertaking international trade, which might result to the expansion of the business in the foreign grounds (Cummings, Bridgman Brown, 2016). On the other hand, Kotters model of change is simply an elaboration of the Lewins model (Refer to Appendice 2). The model helps in the determining of the steps of proper management that is being taken by the organizations in order to bring in modifications in the organizational structure. The urgency of the change that is being faced by the organizations based on the requirements of improvements of the organization and the needs of the consumers is being justified by the model by Kotter. The vision of the change is an important factor that supports the change. The vision of the change is being undertaken after the proper market analysis of the needs. The assessment of the needs helps the organization in determining the backlogs of the organization and thereby helps in bringing in modifications to the specific sections of the organizational structure. Proper communication of the vision helps in undertaking the probable and the most required changes in the system. Communication facilit ates the free flow of the information of the change to the workforce. It is the smooth functioning workforce, which helps the company in undertaking the modifications (Appelbaum et al., 2012). Therefore, communication helps in undertaking steps in order to enhance the capabilities of the company in the market. Empowerment of the workforce helps in the undertaking of the smooth functioning of the organization. Proper motivation of the workers and the gradation and recognition of the efficient functions undertaken by the individuals in the workforce not only motivates the workforce but also makes them efficient through the practices. The model of Kotter helps in the understanding of the necessary steps that the organization must undertake in order to bring in the changes in the structure of the organization. The organizational improvements help in sustaining the organization in the long run. As the model helps in determining the probable steps that must be undertaken by the organizati ons, it becomes easier for the organization to frame their structure likewise and thereby reframe their goals for making the improvements in the organizational structure. The model helps in understanding the totality of the change that is being considered by the organization. The change that is being framed and needed by the organization is firstly analyzed and then supported with a logic for the change. The decisions taken by the management based on the viability of the change helps in the understanding of the progress made by the organization. On the other hand, the vision of the change involves the aspects of creativity. It includes the effective leadership of the organization in order to foresee the requirements of the change that is being faced by the organizations. Implementation of the change in the structure of the organization helps in the understanding of the needs of modifications faced by the organizations (Booth, 2015). Proper implementation of the strategies helps in d etermining the different aspects of the change that is being undertaken by the organization. The very last step undertaken by the companies are similar to the freeze stage of Lewins model. It helps the organization in retaining the changes that are being undertaken in order to bring in modifications in the market of the organization. Implementation of the change and the effects of the undertakings Managing the change helps in the understanding of the future aspects of the organization relating to its progress and growth. Every organization aims at establishing their products and their brands firmly in the market. In order to do so, the companies undertake changes based on the demands of the consumers. It helps in retaining the loyalty of the consumers to the particular brands. Changes are necessary or the better understanding of the situation of the organization and thereby bring in modifications in their structure to improve the situation of the organization in the market structure. Von Krogh (2012) believed that sustenance is the major factor that is being adhered by the companies while they indulge themselves in the assorted change. They customize their policies and bring modifications in their line of products in order to retain the trust and loyalty of the target consumers. The basic consideration undertaken by the organizations are based on the improvements of the situat ion of the organizations in the respective markets. in order to bring in changes in the situation, the companies brings in changes in their processes, which helps them in their sustenance in the market. According to McAfee, Brynjolfsson Davenport (2012), improvements can be settled in the company through the proper functioning of the change management. The decisions taken by the management helps in the under taking of the progress of the organization. Therefore, change management helps in undertaking the most crucial decision based on the improvements of the organizational structure in the market. Delineation of the requirement of the organization and the proper analysis of the change and the effects helps the organization in foreseeing the future prospects of growth and expansion of the organization. The models that are being discussed in the previous section of the report actually helps the entrepreneurs in managing their functions serially and thereby determine the necessity of the change and the kinds of change required by the organization. It helps the organizations in identifying the different aspects of the change that the organization is required to undertake. The enhanceme nt of the teamwork helps in the proficient and smooth functioning of the organization. Organizing the functions of the workforce and the integration of the internal and the external department of the organization helps in undertaking the smooth functioning. It helps in undertaking the progress of the organization based on the requirement of the change. The motivated human resource of the organization helps in undertaking the smooth functioning and thereby proves to be an asset for the organization. The changes undertaken by the organizations help in enhancing the skills of the workforce through proper training of the workforce and the flexibility of the operations undertaken by the workforce (Vaccaro et al., 2012). The envisioned changes help the organization in undertaking steps to formulate strategies that help in enhancing the productivity of the organization and thereby make progression in the market. It also helps the companies in taking the rightful measures based on the requi rements of the change of the organization. According to Cummings Worley (2014), the proper utilization of the resources helps in the undertaking of the progress of the organization in the relative markets. the strategies helps in determining the outputs of the organization and make an increase in the productivity of the organization. proper monitoring over the services and the products manufactured by the organization is being undertaken through the utilization of the factors and the resources. The organization helps in determining the market standards of the organization and thereby brings in changes in the system (Jabri, 2012). Conclusion Therefore, from the above analysis it can be stated that the changes that are being undertaken by the organization and the manner in which the changes affect the situation of the company in the market. The sustainable approach of the organizations is being supported by the changes that the organization aims at undertaking. Adhering to the requirements of the consumers and thereby cope up with the market competition helps in determining the frivolous aspects of the change. It helps the organization sin gaining a competitive advantage in the market. Changes are being induced in the product development stages and the marketing in order to enhance the efficiency of the outputs created by the organizations. The report also discusses the models of change that helps the organizations in determining the steps before they implement the change in their organizational structure. Continuous growth of the organizations helps them in maintaining their existence in the markets. Therefore, the compa ny is required to undertake modifications in order to sustain and grow in the competitive market References Appelbaum, S.H., Habashy, S., Malo, J.L. Shafiq, H., 2012. Back to the future: revisiting Kotter's 1996 change model.Journal of Management Development,31(8), pp.764-782. Booth, S.A., 2015.Crisis management strategy: Competition and change in modern enterprises. Routledge. Cameron, E. Green, M., 2015.Making sense of change management: A complete guide to the models, tools and techniques of organizational change. Kogan Page Publishers. Cummings, S., Bridgman, T. Brown, K.G., 2016. Unfreezing change as three steps: Rethinking Kurt Lewins legacy for change management.human relations,69(1), pp.33-60. Cummings, T.G. Worley, C.G., 2014.Organization development and change. Cengage learning. Doppelt, B., 2017.Leading change toward sustainability: A change-management guide for business, government and civil society. Routledge. Hayes, J., 2014.The theory and practice of change management. Palgrave Macmillan. Hornstein, H.A., 2015. The integration of project management and organizational change management is now a necessity.International Journal of Project Management,33(2), pp.291-298. Jabri, M., 2012.Managing organizational change. Palgrave Macmillan. Kerzner, H., 2013.Project management: a systems approach to planning, scheduling, and controlling. John Wiley Sons. Langley, A., Smallman, C., Tsoukas, H. Van de Ven, A.H., 2013. Process studies of change in organization and management: Unveiling temporality, activity, and flow.Academy of Management Journal,56(1), pp.1-13. Laudon, K.C. Laudon, J.P., 2016.Management information system. Pearson Education India. McAfee, A., Brynjolfsson, E. Davenport, T.H., 2012. Big data: the management revolution.Harvard business review,90(10), pp.60-68. Shirey, M.R., 2013. Lewins theory of planned change as a strategic resource.Journal of Nursing Administration,43(2), pp.69-72. Vaccaro, I.G., Jansen, J.J., Van Den Bosch, F.A. Volberda, H.W., 2012. Management innovation and leadership: The moderating role of organizational size.Journal of Management Studies,49(1), pp.28-51. Von Krogh, G., 2012. How does social software change knowledge management? Toward a strategic research agenda.The Journal of Strategic Information Systems,21(2), pp.154-164.

Isolation and Analysis of Essential Oils Using Gas Chromatography Essay Example

Isolation and Analysis of Essential Oils Using Gas Chromatography Paper Isolation and Analysis of Essential Oils using Gas Chromatography Lyndon Justin T. Guzman Institute of Chemistry, University of the Philippines, Diliman, Quezon City Date Performed: February 2; February 4, 2011 Date Submitted: February 18, 2011 Abstract The purpose of this experiment is to isolate the essential oil from eucalyptus leaves as a pure compound; moreover, the components of the essential oil, camphor and limonene, will be then separated using gas chromatography technique, identify the components by their retention times, and compute for the concentration and percentage content of each component by their peak areas and peak heights. The volatile oil from eucalyptus leaves was isolated with the use of steam distillation setup, then using a separatory funnel to pipette out the extract from the distillate. A gas chromatography, with nitrogen gas as the carrier gas and a flame ionization detector, was used to separate and characterize the components of the essential oil. The retention times, peak areas, and peak heights were obtained for qualitative and quantitative analysis. A percentage of 0. 05% and 2. 85% were obtained as the content of camphor and limonene in the extracted oil, respectively. It also goes to show that limonene has greater concentration than that of camphor in the essential oil extract. Indeed, steam distillation and gas chromatography techniques are essential methods for extracting essential oils and separating natural compounds from plants. I. Introduction Gas chromatography is used for separations of volatile or reasonably volatile organic liquids and solids. In this method of chromatography, the components are partitioned between a liquid coating on the column (the stationary phase) and an inert gas (the mobile phase). We will write a custom essay sample on Isolation and Analysis of Essential Oils Using Gas Chromatography specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Isolation and Analysis of Essential Oils Using Gas Chromatography specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Isolation and Analysis of Essential Oils Using Gas Chromatography specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The stationary phase for gas chromatography is usually an organic polymer coated on the inside of a tube, such as long capillary, and the mobile phase is an inert gas, such as hydrogen, helium, or nitrogen. (Druelinger, 2000) Figure 1. Schematic diagram of a gas chromatographic system. http://www. cee. vt. edu/ewr/environmental/teach/smprimer/gc/gc. html A small volume (1-10 ? L) of a mixture of volatile substances (usually dissolved in a solvent) is injected by syringe onto a heated column through which an inert carrier gas is flowing. The heat applied, as well as the gas flow, helps the molecules from the sample travel through the column. Smaller, more volatile molecules generally emerge first from the opposite end of the column and are detected. The detector is connected to a recorder/data system, which shows a deflection when a sample passes the detector in proportion to the amount of sample detected. Compounds are eluted through an exit port either in an intact form or as combustion products, depending on the type of detector used. (Druelinger, 2000) The characteristic aromas of plants are due to the volatile oils, or also known as essential oils, which have been used since antiquity as a source of fragrances and flavorings. These oils occur in all living parts of the plant; they are often concentrated in twigs, leaves, flowers, and seeds. Essential oils are generally complex mixtures of hydrocarbons, alcohols, and carbonyl compounds mostly belonging to the broad group of plant products known as terpenes. (Dalrymple and Moore, 1976) One of the many types of samples easily characterized by the technique of gas chromatography is the essential oil. These essential oils are isolated from the plant tissue by steam distillation. Since organic compounds are generally miscible with one another, this phenomenon is usually observed only when one of the liquids is water with one or more immiscible organic liquids; in these cases, the distillation process is called steam distillation (Ault, 1983). The technique of steam distillation is based upon the principle that each component of immiscible liquid mixtures contributes to the total vapor pressure as if the other components were not there (Druelinger et. l. , 2000). As the temperature of such a mixture in an apparatus open to the atmosphere is raised, the vapor pressure of each substance increases until the total vapor pressure equals the pressure of the atmosphere. Since the total vapor pressure is the sum of the individual vapor pressures, the total vapor pressure must become equal to atmospheric pressure at a temperature below the boiling point of either pure substance (Ault, 1983). The mixture thus distills at a temperature below the boiling point of either pure component. This can be explained using a combination of Dalton’s and Raoult’s Law: Patm = XAPÂ °A + XBPÂ °B where Patm is the atmospheric pressure, XA and XB are the mole fractions of compounds A and B, and PÂ °A and PÂ °B are the vapor pressures of pure liquids A and B. Their individual contributions are dependent on their respective mole fractions, and both liquids contribute to the vapor pressure of the system (Institute of Chemistry, UPD, 2010). In this experiment, a major constituent of volatile oils from eucalyptus leaves will be isolated as a pure compound with high purity via steam distillation. These essential oils are camphor and limonene and by using the gas chromatography technique, the different components of the eucalyptus essential oil will be separated. This experiment also aims to manipulate the gas chromatography system and change conditions in order to effectively and efficiently separate the components, and therefore achieve a good resolution. The components will be identified by determination of their retention times relative to those of a homologous series of n-alkanes by co-injection with authentic (standard) samples. II. Methodology Extraction of Essential Oils by Steam Distillation A steam distillation setup was prepared as shown in Figure 2 below (Note 1). The sample (Note 2) was cut into small pieces and an amount enough to fill three-fourths of a 1-L round bottom flask was collected. 400-mL distilled water was weighed and added into the flask. The mixture was steam distilled rapidly until you have about 100 mL of the distillate. Figure 2. Steam distillation setup. http://www. pharmainfo. net/reviews/fractional-distillation-binary-solvent-mixture The distillate was placed in a separatory funnel and 2. 0 g NaCl was added. The funnel was left to stand until separation of layers occurred. All the extract was then pipetted out (Note 3). The mixture was dried by adding enough anhydrous sodium sulfate to the mixture until the sodium sulfate swirled freely. If the entire drying agent clumped, another spatula-full anhydrous sodium sulfate was added. The mixture was then swirled. The mixture was dry if there were no visible signs of water and the drying agent flowed freely in the container. The essential oil and aqueous distillate were stored in separate tightly-sealed, properly-labeled containers (vials) in the freezer for future use in the FT-IR analysis experiment or GC experiment or special project (Note 4). Gas Chromatographic Analysis of Essential Oils Solution Preparation 1. Reference Solution. Separate stock solutions of 500 ppm of camphor and limonene in acetone were prepared. 50-300 ppm working standard solutions were also prepared (50, 100, 150, 200, 250 ppm) (Note 5). 2. Essential Oil Extract. 0. 5 mL of the pure extract (from steam distillation) was measured and diluted with acetone in a 10-mL volumetric flask. Instrumentation Gas chromatography was performed using a Shimadzu GC-14B using Equity 1 (30 m x 0. 25 ? m ID, 0. 25 mm film thickness) capillary column with N2 as a carrier gas. The following were the operating conditions: N2 flow rate| 1. 0 mL/min| Column temperature| initial 50Â °C (at 4 mins)| Ramp| 20Â °C/min to 210Â °C| Injector temperature| 200Â °C| Detector temperature| 250Â °C| Before the start of the experiment, the GC must have warmed up. Refer to the GC condensed procedure. Gas Chromatographic Analysis 1. 0 ? L of the standard camphor solution was injected and its chromatogram was generated. The procedure was repeated using limonene standard solution. The recorded retention times and peak areas of these substances were noted. 1. 0 ? L of the test solution (essential oil extract) was injected. Using the retention times determined from the chromatogram with the standard solution, the components of the standard solution was located on the chromatogram obtained with the test solution (Note 6). After all solutions have been injected and data computer-processed, the GC was left to run at the highest column temperature used in the experiment with the N2 gas flowing at a slower rate than the experimental flow rate for 15-20 minutes. The injector temperature was set to room temperature and slowly lowered the column temperature down to room temperature with the N2 gas still flowing in the system. Once everything reached room temperature, the gas flow was left to stand for another 10-15 minutes, after which the GC can be turned off. Notes 1. Boiling chips were added to the steam generator and sample flasks. 2. Each group must use different plant samples. 3. There should be two layers after the addition of NaCl solution. One was mostly water. The other was mostly extracted oil. To find out which is which, a small amount of water was added to the flask, whichever layer dissolved the water drop was the aqueous layer. The layer of essential oil was then carefully pipetted out. . If you have to get more of the organic layer out of the water, you can perform a back-extraction (solvent extraction) experiment. 5. All standard and sample solutions were stored in a well-filled, airtight container, protected from light and a temperature not exceeding 25Â °C. 6. The assay was not valid unless the number of theoretical plates calculated for the peak due to limonene at 110Â °C was at leas t 30000; the resolution between the peaks corresponding to limonene and cineole was at least 1. 5. Waste Disposal All solid wastes were disposed in the trash can. Waste acetone was poured into properly labeled waste container exclusively for acetone. Do not pour waste acetone in the sink! III. Results and Discussion The extraction of the essential oils, camphor and limonene, from the eucalyptus leaves sample was carried out using the steam distillation technique. camphor Figure 3. Structural formulas for camphor and limonene. The boiling point of the oily, aqueous distillate will never exceed the boiling point of water. This is because both water and the oily component each contribute to the total vapor pressure as if the other component was not present. The mixture boils when the combined vapor pressures of water and oil equal the atmospheric pressure. The oil has a small, but significant vapor pressure at 100Â °C, so that the boiling point of the mixture will be just below the boiling point of water. (Druelinger, 2000) The mass of the eucalyptus leaves that were extracted was 112. 98 g. 100 mL of the distillate was produced from the steam distillation. Only a small amount of oil was extracted within the distillate by a separatory funnel. The components of the oil sample were then separated and characterized using the gas chromatography technique with a flame ionization detector. Nitrogen gas served as the carrier gas or the mobile phase that moved the sample throughout the column. The chromatograms, plots of detector response versus time, of the standards and the sample were taken. Retention times were noted for qualitative analysis. Peak areas and peak heights were also recorded for and quantitative analysis of the essential oils. Table 1. Retention times of camphor and limonene standard and sample solutions. Solution| Retention Time (min)| pure standard camphor| 9. 021| pure standard limonene| 7. 908| extracted camphor sample| 9. 347| extracted limonene sample| 7. 89| From the given data above for the retention time, the retention time of the camphor and limonene from the standard solutions, 9. 021 min and 7. 908 min, respectively, were close to the retention time of the camphor and limonene with that of the sample solution, 9. 347 min and 7. 889 min. It was deduced that the camphor and limonene from the sample solution were present compounds in the extrac ted oil from eucalyptus leaves. Below is a table shown for the resulted peak areas and peak heights from the chromatograms of camphor and limonene standard solutions. Table 2. Peak areas and peak heights of camphor and limonene standard solutions. Solution| Peak Area| Peak Height| pure standard camphor| 46848| 17109| 50 ppm| 4427| 1113| 150 ppm| 12904| 4627| 200 ppm| 20417| 6065| 250 ppm| 15683| 5282| pure standard limonene| 56156| 20681| 50 ppm| 4419| 1236| 150 ppm| 15058| 4946| 200 ppm| 20464| 6452| 250 ppm| 20875| 5913| Figure 4. Camphor standard solutions vs. Peak area. Figure 5. Camphor standard solutions vs. Peak height. Table 3. Determination of the concentration of camphor component in the sample. Camphor Sample| Value| Concentration (ppm)| Peak Area| 5820| 67. 8| Peak Height| 1544| 61. 57| From the plotted calibration curve on the peak height and peak area for the camphor component, a regression equation is formulated in each curve with linearities almost equal to 1. From the acquired data on peak area and peak height of the camphor sample, the concentration of the camphor is 67. 98 ppm when the peak area is 5820 and 61. 57 ppm when the peak heigh t is 1544. Figure 6. Limonene standard solutions vs. Peak area. Figure 7. Limonene standard solutions vs. Peak height. Table 4. Determination of the concentration of limonene component in the sample. Limonene Sample| Value| Concentration (ppm)| Peak Area| 306384| 2875. 39| Peak Height| 102881| 2943. 95| From the plotted calibration curve on the peak height and peak area for the limonene component, a regression equation is formulated in each curve with linearities also almost equal to 1. From the obtained data on peak and peak height of the limonene sample, the concentration of the limonene is 2875. 39 ppm when the peak area is 306384 and 2943. 95 ppm when the peak height is 102881. To determine the percentage content of the components of the essential oil, the area normalization method is applied. Determining the areas beneath all of the peaks of a chromatogram enables to assign percentages to each of the components of a sample. Table 5. Determination of the percentage content of camphor and limonene sample. Component| Area| Total Area| % Content| camphor| 5820| 10766407| 0. 05%| limonene| 306384| | 2. 85%| Using the formula for area normalization, the computed percentage contents for camphor and limonene are 0. 05% and 2. 85%, respectively. This suggests that there is a greater amount of limonene in the oil extracted from the eucalyptus leaves than that of camphor. The very low percentage implies that extracting and separating natural organic compounds from essential oils give you a very low yield that’s why you need to have huge amounts of starting material to extract from to get a relatively high percentage of its components. IV. Conclusion In this experiment, the essential oil from eucalyptus leaves was isolated as a pure compound by steam distillation. The components of the eucalyptus essential oil, camphor and limonene, were separated using the gas chromatography technique having a flame ionization detector. The components were also identified through determination of their retention times and were confirmed that camphor and limonene are present, having a retention time of 9. 347 and 7. 889, respectively. Calibration curves on peak areas and peak heights on camphor and limonene were formed. Concentrations of the components were calculated and gave 67. 98 ppm and 61. 57 ppm for camphor, and 2875. 39 ppm and 2943. 95 ppm for limonene. The percentage contents of the components were also determined. The essential oil extracted constituted 0. 5% camphor and 2. 85% limonene. Steam distillation is a useful method for isolating high-boiling liquids, such as oils, from other non-volatile organic compounds, such as waxes, complex fats, proteins, and sugars (Druelinger, 2000). Natural oils can be isolated readily by steam distillation. Individual compounds can be separated from the essential oil by gas chromatography wherein the components of a vaporized sample are separated as a consequence of bei ng partitioned between a mobile gaseous phase and a liquid stationary phase held in a column. Gas chromatography is the most widely used technique for qualitative and quantitative analysis for analysis times are short, very small amounts of sample are required and an ideal tool for the microscale and miniscale organic laboratories. If you want to obtain large percentage of compounds from the extracted essential oil, you need to have huge amounts of eucalyptus leaves and it will take you a long time to steam distill. The standards should be injected under the same set of conditions for if not, this will lead to erroneous comparisons of data. You can manipulate some parameters like type of column, carrier gas flow rate, injector temperature, and column temperature to compare some effects on the quantitative breakdown of the experiment. V. References Skoog, D. A. , West, D. M. , Holler, F. J. and S. R. Crouch. 2004. Fundamentals of Analytical Chemistry, 8th edition. Thomson Learning Asia, Singapore. Institiute of Chemistry. Intermediate Chemistry Laboratory II Manual. 2010. University of the Philippines, Diliman, Philippines. Druelinger, M. L. , B. A. Gaddis and A. M. Schoffstall. 2000. Microscale and Miniscale Organic Chemistry Laboratory Experiments. The McGraw-Hill Companies, Inc. , USA. Dalrymple, D. L. and J. A. Moore. 1976. Experimental Methods in Organic Chemistry, 2nd edition. W. B. Saunders Company, USA. Ault, A. 1983. Techniques and Experiments for Organic Chemistry, 4th edition. Allyn and Bacon, Inc. , USA. VI. Appendix Calculations Concentration of camphor component in the sample peak area = 5820 regression equation: y = 103. 48x – 1214. 9 5820 = 103. 48x – 1214. 9 x = 67. 98 ppm peak height = 1544 regression equation: y = 33. 317x – 507. 9 1544 = 33. 317x – 507. 29 x = 61. 57 ppm Concentration of limonene component in the sample peak area = 306384 regression equation: y = 106. 88x – 937. 57 306384 = 106. 88x – 937. 57 x = 2875. 39 ppm peak height = 102881 y = 35. 106x – 469. 43 102881 = 35. 106x – 469. 43 x = 2943. 95 ppm Percentage content of camphor sample %content = (area / total area) x 100 %co ntent = (5820/10766407) x 100 %content = 0. 05% Percentage content of limonene sample %content = (area / total area) x 100 %content = (306384/10766407) x 100 %content = 2. 85% Answers to Questions 1. The gas chromatography technique is used for separations of volatile or reasonably volatile organic liquids and solids. 2. Thermal conductivity detectors (TCD), flame ionization detectors (FID), and electron capture detectors (ECD) are commonly used type of detectors. The thermal conductivity detector, which was one of the earliest detectors for gas chromatography, senses a difference in thermal conductivity of gases eluting from a GC column. The thermal conductivities of helium and hydrogen are roughly 6 to 10 times greater than those of most organic compounds. Thus, even small amounts of organic species cause relatively large decreases in the thermal conductivity of the column effluent, which results in a marked rise in the temperature of the detector. (Skoog et. al. , 2004) Flame ionization detectors, the most widely used and applicable detector for GC, consist of a flame fueled by hydrogen gas. Functional groups, such as carbonyl, alcohol, halogen, and amine, yield fewer ions or none at all in a flame. The detector is insensitive towards non-combustible gases such as H2O, CO2, SO2, and NO2. These properties make the FID a most useful detector for the analysis of most organic samples, including those that are contaminated with water and the oxides of nitrogen and sulfur. (Skoog et. al. , 2004) The electron capture detector has become one of the most widely used detectors for environmental samples because this detector selectively responds to halogen-containing organic compounds, such as pesticides and polychlorinated biphenyls. (Skoog et. al. , 2004) 3. An elution with a single solvent or a solvent mixture of constant composition is isocratic. For samples with a broad boiling range, it is often desirable to employ temperature programming, whereby the column temperature is either increased continuously or in steps as the separation proceeds. 4. polydimethyl siloxane – general-purpose nonpolar phase, hydrocarbons, polynuclear aromatics, steroids, PCBs polyethylene glycol – free acids, alcohols, ethers, essential oils, glycols 5% phenyl-polydimethyl siloxane – fatty acid methyl esters, alkaloids, drugs, halogenated compounds 50% cyanopropyl-polydimethyl siloxane – polyunsaturated fatty acids, rosin acids, free acids, alcohols . Table 6. Internal Standard method for GC. % analyte| Peak height analyte| Peak height internal std| Peak height ratio (analyte/internal std)| 0. 05| 18. 8| 50. 0| 0. 38| 0. 10| 48. 1| 64. 1| 0. 75| 0. 15| 63. 4| 55. 1| 1. 15| 0. 20| 63. 2| 42. 7| 1. 48| 0. 25| 93. 6| 53. 8| 1. 74| unknown| 58. 9| 49. 4| 1. 19| Figure 8. Peak Height Ratio vs. Percent Analyte. regression equat ion: y = 6. 9x + 0. 065 slope = 6. 9 y-intercept = 0. 065 concentration of unknown: . 19 = 6. 9x + 0. 065 x = 0. 16304 standard deviation = 0. 007939 Chromatograms Figure 9. 50 ppm standard solution chromatogram. Figure 10. 150 ppm standard solution chromatogram. Figure 11. 200 ppm standard solution chromatogram. Figure 12. 250 ppm standard solution chromatogram. Figure 13. Pure standard camphor solution chromatogram. Figure 14. Pure standard limonene solution chromatogram. Figure 15. Essential oil extract chromatogram.